The growth of Sal. choleraesuis and its kunzendorf variety in tetrathionate broth containing various amounts of iodine solution was studied and compared with that of Sal. typhi, Sal. typhimurium and E. coli. The results obtained were as followings.
1. When 2.0 ㎖ of iodine solution, normal amount, was added to 100 ㎖ of tetrathionate broth base, the number of Sal. choleraesuis decreased rapidly until 24 hours after inoculation and slightly increased 48 hours after inoculation. The numbers of Sal. choleraesuis var. kunzendorf and E. coli decreased rapidly and none of the organisms recovered 24 and 48 hours after inoculation, respectively. The growth of Sal. typhi and Sal. typhimurium, however, was not inhibited at all.
2. When 4.0 ㎖ of iodine solution was added, to 100 ㎖ of tetrathionate broth base, the growth of all the organisms was inhibited, among which Sal. choleraesuis, Sal. choleraesuis var. kunzendorf, and E. coli were not recovered 24 and 48 hours after inoculation.
3. When reduced amounts of iodine solution, 1.0 ㎖ and 0.5 ㎖, were added to 100 ㎖ of tetrathionate broth base, the growth of all the organisms was not inhibited.
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